Securing first place is Febby Mpundu, a 20 year old Zambian born artist studying Fine Arts at Oxford Brookes University.
Febby impressed the judges with the moody fluidity of the photographic image she created, taking inspiration from La Traviata's tragic heroine Violetta.
Febby said: "I feel honoured to win and think it is great that Glyndebourne encourage young, new artists in this way.
"I like my work to reflect a person's character through colour or motion and I enjoy creating portraits that focus on expression and the features of an individual.
"A single face has a great way of captivating attention."
The standard of submissions for the competition was incredibly high; art students, designers and working artists from across the UK submitted outstanding pieces inspired by Verdi's La traviata,
one of three Glyndebourne
operas that will tour the country from October to December.
UK-based Febby will see her artwork grace the cover of 10,000 copies of the Glyndebourne Tour programme.
The Tour art competition launched in 2013 and was created in response to the success of Glyndebourne's sister publication, the Festival Programme book, a collector's item renowned for its covers by established artists such as: Julian Opie, Michael Craig Martin, David Hockney and Grayson Perry.
Securing second place is Svitlana Biedarieva. Born in Ukraine, Svitlana, 26, is a London-based artist currently working on her PhD at the Courtauld Institute of Art. Svitlana"s work incorporates the idea of touring opera across the country.
As well as exhibiting work in the UK, Svitlana has also been involved in exhibitions in Germany, Mexico, Estonia, Ukraine and Finland.
Falmouth University graduate Alex Jabore secured third place in the competition.
Alex, 27, has a degree in Fine Art and specialises in traditional oil painting. Alex is London-based and is passionate about opera.
Her work entitled The Finale focuses on Violetta's death and its tragic finality.
In the spirit of Glyndebourne's Tour, which has championed young, emerging operatic talent since its advent in 1968, entrants were restricted to those under the age of 30.
Glyndebourne"s ethos of developing emerging talent. Gus Christie, Glyndebourne Chairman said:
"Visual arts have always been an integral part of Glyndebourne on and off the stage and we hope that by encouraging artists from across the UK to create work inspired by our productions, we are providing an opportunity and a platform for young visual artists to grow and develop their portfolio of work.
"The standard of submissions in 2014 has been incredibly high; we are delighted so many people have been inspired to create work for this competition, only in its second year."
Since the advent of the Tour in 1968, Glyndebourne has sought to broaden the reach of opera, bringing the art form to new audiences every autumn as it takes three productions on the road.
The Tour Art competition extends the reach of opera once again and allows Glyndebourne to champion young and emerging artistic talent of a different kind.
Glyndebourne will launch its 2014 Tour on October 4 and the winning artists will see their work exhibited in the Stalls Gallery at Glyndebourne from 4-26 October 2014.