Café Domenica at Brighton Dome will be based in the venue's Foyer on Church Street offering visitors a selection of freshly made food and drinks four days a week.
Team Domenica was set up to offer people with learning disabilities the opportunity to reach their full potential through work-based training.
Due to social distancing measures, Team Domenica's two Brighton & Hove locations have had to reduce their opening hours and training schedules and needed an additional site to continue their work with candidates.
Rosa Monckton, Team Domenica Founder & Chairman said:
"The café will be a perfect training facility for our candidates and we are grateful to be able to use the space to increase the enrichment opportunities which are a crucial part of their personal development programme."
Brighton Dome closed to the public in March 2020 due to the coronavirus pandemic.
The venue will implement COVID-secure measures including additional cleaning and social distancing to protect staff and visitors to the café.
Andrew Comben, Chief Executive, Brighton Dome added:
"We are delighted to be able to open our doors again to support Team Domenica's valuable work. Brighton Dome exists for people to enjoy themselves and this is a positive first step to welcoming visitors into the venue as we start to plan bringing live events back to the heart of the city.
"Our staff look forward to working with Team Domenica candidates on developing their skills to create positive outcomes for their future careers."
Profits from café sales go towards supporting the work of the charity.
Team Domenica candidate Olivia Gordon who will be training at the café said:
"I am feeling really really excited. I love being at the Dome. I did my work placement and ushering there and can't wait to go back and work in the Team Domenica Café."
Café Domenica at Brighton Dome, Church Street, Brighton BN1 1UE opens from 15 September 2020, Tuesday to Friday, 9am to 4pm.